ezPATCH 100A Smart Manipulator
The ezPatch 100A is a smart manipulator. It has all the functions a manual manipulator has.
In addition, it comes with a computer controlled feedback system so that it can stop precisely after
the electrode touches the cell membrane. The system is ideal for patch clamping.
In patch clamp experiments, all users need to do is to aim the electrode at a selected cell.
A click of a button and the electrode moving at a specified speed will stop at the surface of the
cell membrane at specified pressure (measured by resistance increase). If used with our
ez-gSEAL pressure controller, suction will be automatically appled to make seal and suction pulses
will be applied for going whole cell. Patch clamp experiments will be consistent and
precise once you find the best seal parameters. The seal parameters can
be saved for different cell types and shared among lab members.
The control software has many functions that make patch clamp experiments easy and precise.
You can excise patches at a predefined speed. With the manual control functions
in the software, you can move the electrode forwards or backwards in as small as 0.1 µm steps at a defined speed.
The software can also control multiple manipulators. Therefore, users can make seals on the
same cell or a few different cells simultaneously.
If drift from the pipette holder/o-ring is a concern, an optional pipette-holder clamp can be purchased.
It clamps the glass pipette so that the drift caused by an o-ring is minimal.
There is no additional electrical noise from the manipulator since no current is
going through the motor when it stops. The friction ensures that the motor
does not lose even a single micro step of the gear (50 nm).
The BNC interface box comes with 10 analog inputs and 2 analog outputs. An output trigger
is provided for synchronization in case it is needed. For the best grounding results, software
controls the BNC interface box to be either in a float or grounded condition.
The ezPATCH 100A manipulator comes with National Instruments' PCI 6221 DAQ board, which is supported by many data
acquisition programs such as jCLAMP. If you plan to use
such software you will not need to purchase an extra data acquisition board.
Key Features
- Includes a computer-controlled manipulator, a data acquisition board, a BNC interface box, and control software.
- Resolution of the computer controlled manipulator is 50 nm.
- Manipulator movement stops when patch pipette touches the cell membrane.
- No electrical noise during recording.
- Ideal for patch clamping.
System Specifications
Major Components
Motorized Stage
|
Manual XY Stage
Data Acquisition Board
|
Computer Requirements
- Windows 2000 or XP with 2.0 GHz CPU
- At least 1 USB port
- 1 PCI slot
US Price
$8,500.00
Contact us for international pricing.